Optimized fusion product to transfer siRNA into living cells for rapid and efficient gene silencing
- Exceptionally efficient siRNA transfection, especially in primary and non-dividing cells, such as keratinocytes
- Extremely low cytotoxicity
- Fastest siRNA delivery—no endocytosis or lysosomal degradation
Technical Features
- Lipofection-independent transfer of siRNA into living cells
- Fast siRNA transfection into the cytoplasm: no endocytosis, no lysosomal degradation, and no gene transfer to the nucleus
- siRNA transfer completed within 5-20 minutes
- Small amount of siRNA is sufficient for effective knockdown
- Excellent biocompatibility because of low cytotoxicity
- siRNA transfer into cells without generating genetically modified organisms (GMO)
Applications
- Gene silencing / Protein knockdown
Fuse-It-siRNA Results in Extremely Fast siRNA Transfer and Highly Efficient Knockdown of GFP
Transiently GFP-expressing CHO-K1 cells were fused with Fuse-It-siRNA vesicles containing Silencer® GFP (eGFP) siRNA (Thermo Fisher Scientific) for 8 minutes. In contrast to the control cells, GFP was almost completely diminished in Fuse-It-siRNA-treated cells 18 hours after the transfer.
Membrane Fusion – The Direct Path to Gene Silencing
Ordering information
| Cat. No. | Description | Net price excl. value added tax | |
|---|---|---|---|
| 60510 | Fuse-It-siRNA, infrared fluorescent: 2 x 150 µl solution | 278 € | |
| 60511 | Fuse-It-siRNA, infrared fluorescent: 2 x 300 µl solution | 483 € |