Optimized product to incorporate lipids or amphipathic molecules into the plasma membrane of living cells by fusion
Technical Features
- Transfer of lipids and amphipathic molecules into the cell plasma membrane in living cells by the use of fusogenic liposomes
- Superb fusion efficiency of 80 to 100% independent of the cell type used
- Fusion process completed within 1 to 10 minutes with superior biocompatibility
Applications
- Lipid imaging for turnover, traffic, and functional analyses in living cells
- Lipid raft, lipid microdomain, and lipid diffusivity measurements in living cells
- Blockade, induction, or replacement of lipid induced/regulated signal cascades
- Re-incorporation of lipids into mutant cells whose lipid biosynthesis is altered
The cells can be analyzed immediately after fusion has taken place. If necessary more than just one lipid of interest can be incorporated at once. Depending on experimental needs, fusion can be detected by fluorescence.
Bodipy FL C12-galactocerebroside and TopFluor cholesterol were incorporated into the plasma membrane of CHO cells using Fuse-It-L after 5 minutes of incubation.
Ordering information
| Cat. No. | Description | Net price excl. value added tax |
| 60210 | Fuse-It-L, lyophilized, for 100 μl solution, IR fluorescent | 193 € |
| 60211 | Fuse-It-L, lyophilized, for 4 x 25 μl solution, IR fluorescent | 217 € |
Specifications/Content
Concentration 3 mM
Storage -20°C
Shelf life 6 months as solution
ExMax/EmMax 750/780 nm