A fusion reagent for rapid mRNA transfection into the cytoplasm of living cells
Technical Features
- Immediate mRNA translation leads to protein synthesis detectable after only 15-30 minutes
- Beniag’s fusion mechanism works perfectly in proliferating cells as well as in non-proliferating cells (e.g. cardiomyocytes)
- Highly efficient and biocompatible, especially in primary cells (e.g., neurons or HUVECs) and stem cells
Applications
- mRNA translation and degradation studies
- Protein biochemistry studies: synthesis, folding, processing, stability, localization, degradation
- mRNA transfer into primary cells without generating genetically modified organisms (GMO)
- Genome engineering by RNA-only CRISPR/Cas technology
Technical Features
- Lipofection-independent transfer of mRNA into living cells
- the transfer of mRNA is completed within 5–20 minutes
- No endocytosis involved
- No lysosomal degradation needed
- without transfer to the nucleus
- Protocol optimized for the transfer of functionally capped and polyadenylated mRNA
- No Biosafety Laboratory Level 1 or 2 necessary
- Excellent biocompatibility with low cytotoxicity
Membrane Fusion – The Direct Way to Protein Expression
The novel mRNA transfection reagent Fuse-It-mRNA uses fusogenic liposomes for mRNA transfer without endosomal release
Efficiency of mRNA Expression in Various Cell Types After Treatment with Fuse-It-mRN
Celltype overview
| Cell Type | Organism | Incubation Time | Efficiency of mRNA Expression* |
| Primary Cells | |||
| nHEK, foreskin | human | 8 min | 70–90% |
| Cortical neurons, embryonic (adherent) | rat | 10 min | 40–60% |
| HFF | human | 10 min | 50–70% |
| Cell Lines | |||
| A549 | human | 10 min | 80–100% |
| CHO-K1 | hamster | 10 min | 80–100% |
| Beas-2B | human | 8 min | 80–100% |
| 3T3 | murine | 8 min | 80–100% |
| MEF wildtype | murine | 10 min | 70–90% |
| HT-1080 | human | 10 min | 80–100% |
| HUH-7 | human | 8 min | 70–90% |
| MCF-10A | human | 10 min | 70–90% |
| Rat1 | rat | 10 min | 80–100% |
| THP-1 (suspension) | human | 3 min | 70–90% |
* The mRNA expression efficiency may vary, depending on further experimental factors (e.g., culture medium, cell fitness, passage number, and protocol optimization).
Citations and references
Smuggling on the Nanoscale—Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo
Ordering information
| Cat. No. | Description |
Net price excl. value added tax |
|
|---|---|---|---|
| 60505 | Fuse-It-mRNA, infrared fluorescent: 2 x 150 µl solution | 278 € | |
| 60506 | Fuse-It-mRNA, infrared fluorescent: 2 x 300 µl solution | 483 € |